Cell Based Assays
Chrono Log Corporation CHRONO-PAR Collagen Reagent, High Concentration Platelet Aggregation Reagent, Validated for Whole Blood and PRP, 1 mL
The CHRONO-PAR Collagen Reagent is a highly concentrated platelet aggregation reagent that enables researchers to conduct detailed platelet function and secretion studies. It efficiently activates platelets with 1 mg of native Type I collagen sourced from equine tendons. This versatile reagent is validated for use with various blood samples, making it essential for diagnosing platelet dysfunctions and confirming normal platelet function in diagnostic applications.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis Premium kit (E. coli CFPS Premium): 1 mL
Ginkgo's CFPS Premium kit is Ginkgo's premier cell free protein synthesis (CFPS) product, optimized for the highest yields and most challenging proteins. This E. coli transcription/translation system is optimized to produce proteins from a T7 RNA Polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user simply provides a DNA template encoding the protein of interest under control of a T7 promoter. The kit also contains a Solubility Enhancer that can help improve expression and folding of challenging protein targets. Contents: E. coli lysate, Reagent Mix (3.3x), Solubility Enhancer (10x), and control plasmid. The 1 mL kit enables 50 reactions of 20 µL, or reaction volumes can be scaled to your specifications. Storage at -80° C. For research use only.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 1 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 100 mL kit enables user to run 5000 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Dojindo Molecular Technologies Inc ROS Assay Kit -Highly Sensitive DCFH-DA- (100 tests) allows high-sensitivity detection of total ROS with fluorescence microscopes, plate readers or flow cytometers (Ex: 505 nm/Em: 525 nm) 100 tests
Reactive oxygen species (ROS) are primarily produced in mitochondria during ATP synthesis. ROS play an essential role in signaling pathways and the immune system, whereas increasing ROS is associated with diseases and cellular senescence. Recent studies suggested that ferroptosis is a new type of cell death characterized by iron dependency and increasing ROS. Thus, ROS detection has been attracting considerable interest in ferroptosis research. DCFH-DA is widely used for ROS detection, but it has some limitations like weak fluorescence signals and high background. Dojindo’s ROS Assay Kit -Highly Sensitive DCFH-DA- overcomes these limitations. The dye employed in the kit allows ROS detection with higher sensitivity than DCFH-DA. Moreover, the Loading Buffer included in this kit maintains cellular health during assays. Dojindo has various reagents for ferroptosis research. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Abcam Cell Counting Kit 8 (WST-8 / CCK8)
Cell Counting Kit 8 (WST-8 / CCK8)
The product is subject to the following: Abcam Restricted Use Statement
Biosurface Technologies Corp MicroWell Biofilm Plate Assay, for Rapid Biofilm Testing, Annodized Aluminum, fits most standard 48-well plates.
"The MicroWell™ Biofilm Plate Assay holds 48 coupons, each measuring 0.25 inches by 0.80 inches, inside the wells. This versatility allows researchers to choose from a wide variety of materials, including custom options upon request, to tailor their studies to specific needs.Sterility and convenience are at the forefront of the MicroWell™ assay’s design. The system utilizes the disposable lid that comes with 48-well plates, fitting securely over the coupon holder plate to prevent contamination during incubation. Additionally, the anodized aluminum plate is fully autoclavable, allowing for the reuse of the system. This feature ensures long-term reliability and promotes cost-efficiency and environmental sustainability by reducing the need for disposable components.Designed to facilitate biofilm assays similar to the Calgary Biofilm Device using ASTM E2799*, the MicroWell™ system offers expanded material choices.
Cellecta Inc LentiTrans™ Transduction Reagent (Polybrene for Transduction)
Cellecta’s LentiTrans™ Lentiviral Transduction Reagent is a pre-formulated, easy to use polybrene solution to maximize transduction of any lentivirus. Just add a few microliters to your media during viral inoculation. No preparation necessary. Polybrene is a cationic polymer that enables viral particles to interact more easily with the cell surface. A small amount of the Lenti-Transduction Reagent, which is innocuous to most cell types, into the media during transduction improves rates viral uptake significantly.
Abcam WST-1 Assay Kit (Cell Proliferation)
WST-1 Assay Kit (Cell Proliferation)
The product is subject to the following: Abcam Restricted Use Statement
Lonza Walkersville hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium
Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKit Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin. Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKit Medium*: Human bone marrow derived stem cells Human umbilical cord blood derived stem cells Human umbilical cord matrix derived stem cells C57BL/6 mice bone marrow derived stem cells human adipose derived stem cells Human hamstring tendon derived stem cells.
